한국생물공학회:학술대회논문집
- 2002.04a
- /
- Pages.257-260
- /
- 2002
Production of tissue-type plasminogen activator from immobilized CHO cells introduced hypoxia response element
- Bae, Geun-Won (Graduate school of Biotechnology, Korea Univ.) ;
- Kim, Hong-Jin (Microbiology Section, College of Pharmacy, Chung Ang Univ.) ;
- Kim, Gi-Tae (Seoulin Bioscience Institute) ;
- Kim, Ik-Yeong (Seoulin Bioscience Institute)
- Published : 2002.04.12
Abstract
Dissolved oxygen level of cell culture media has a critical effect on cellular metabolism, which governs specific productivity of recombinant proteins and mammalian cell growth However, in the cores of cell aggregates or cell-immobilized beads, oxygen level frequently goes below a critical level. Mammalian cells have a number of genes induced in the lower level of oxygen, and the genes contain a common cis-acting element (-RCGTG-), hypoxia response element (HRE). By binding of hypoxia inducible factor-l (HIF-I) to the HRE, promoters of hypoxia inducible genes are activated, which is a survival mechanism. In this work, to develop a CHO cell capable of producing recombinant proteins in immobilization and high density cell culture efficiently, mammalian expression vectors containing human tissue-type plasminogen activator (t-PA) gene controlled by HRE were constructed and stably transfected into the CHO cells. In
Keywords