Does Agitation Condition Affect the Correlation Between in vitro Permeability of Xenobiotics across Caco-2 Cells and in vivo Bioavailability of the Compounds\ulcorner

Caco-2 is a cell line derived from the human colon adenocarcinoma and often used as a model for studying intestinal drug absorption. It has been well-known that a strong correlation holds between in vitro permeability across Caco-2 cell monolayers and in vivo bioavailability for various drugs. but the correlation curves varied depending on laboratories. The permeabilities of drugs across Caco-2 cell monolayers have been measured under different agitation conditions. (omitted)