Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2002.10a
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- Pages.415.3-416
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- 2002
Preparation and stability of N-terminal PEGylated Recombinant Human Epidermal Growth Factor
- Na, Dong-Hee (Drug Targeting Laboratory, College of Pharmacy, SungKyunKwan University) ;
- Youn, Yu-Seok (Drug Targeting Laboratory, College of Pharmacy, SungKyunKwan University) ;
- Park, Chong-Jeon (Drug Targeting Laboratory, College of Pharmacy, SungKyunKwan University) ;
- Lee, Sang-Deuk (Drug Targeting Laboratory, College of Pharmacy, SungKyunKwan University) ;
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Lee, Kang-Choon
(Drug Targeting Laboratory, College of Pharmacy, SungKyunKwan University)
- Published : 2002.10.01
Abstract
To improve the stability of recombinant human epidermal growth factor (rhEGF) as therapeutic agent. the N-terminal PEGylated rhEGF (N-PEG-rhEGF) was prepared by site-specific bioconjugation and the stability was investigated in rat skin wound homogenates. Two different N-PEG-rhGEFs (N-PEG5K- and N-PEG20K-rhEGF) were successfully prepared with the yields of above 70%. The PEGylation site was directly confirmed by determining the molecular mass of Lys-C digested samples using MALDI- TOF MS. (omitted)
Keywords