Proceedings of the PSK Conference (대한약학회:학술대회논문집)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Simultaneous quantitation of enalapril and enalaprilat in human plasma by high-throughput solid phase extraction and liquid chromatography/tandem mass spectrometry

  • Son, Jung-Hyun (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology) ;
  • Lee, Jae-Ick (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technolog) ;
  • Kim, Dong-Hyun (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology)
  • Published : 2002.10.01
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Abstract

Enalapril (ENP) maleate is effective drug for the treatment of renivascular hypertension and heart failure. ENP acts as inhibitor of the enzyme angiotensin-convertase (ACE-inhibitor) and metabolized to enalaprilat (ENPT), which is the active metabolite that is really responsible for the therapeutic action. In the present study, a sensitive and rapid liquid chromatography/ electrospray ion trap tandem mass spectrometry (LC/MS/MS) method combined with high-throughput solid phase extraction (SPE) has been developed and validated for the simultaneous quantitative determination of ENP and ENPT in human plasma. (omitted)

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