Proceedings of the PSK Conference (대한약학회:학술대회논문집)

The Pharmaceutical Society of Korea (대한약학회)
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Cloning. Sequencing and Characterization of the Novel Penicillin G Acylase Gene from the Soil-isolated Leclercla adecarboxylata

  • Jun , Sang-O. (Catholic Univ. of Daegu, College of Pharmacy) ;
  • Lim, Ho-S. (Catholic Univ. of Daegu, College of Pharmac) ;
  • Kim, Geun-Y. (Catholic Univ. of Daegu, College of Pharmac) ;
  • Lee, Eung-S. (Catholic Univ. of Daegu, College of Pharmac) ;
  • Lee, Mann-H. (Catholic Univ. of Daegu, College of Pharmacy)
  • Published : 2002.10.01
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Abstract

A novel penicillin G acylase (PGA)-producing bacterial strain was isolated from soil by using the Serratia marcescens overlay technique. The isolated strain was identified as Leclercia adecarboxylata based on the analyses of the biochemical characteristics (API 20E). the cellular fatty acid profile. and the 16S rDNA sequences. The gene encoding the PGA (pac gene) was cloned into the pHSG399 vector and the recombinant E. coliHB101 clones harboring the pac gene were isolated on agar plates containing phenylacetyl-L -leucine and penicillin G. (omitted)

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