Proceedings of the PSK Conference (대한약학회:학술대회논문집)

The Pharmaceutical Society of Korea (대한약학회)
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An In Vitro Bioassay for Nerve Growth Factor

  • Choi, Young-Ju (Division of Biotechnology, Biologics Evaluation Department, Korea Food and Drug Administration) ;
  • Kim, Seon-Mi (Division of Biotechnology, Biologics Evaluation Department, Korea Food and Drug Administratio) ;
  • Park, Sun-Young (Division of Biotechnology, Biologics Evaluation Department, Korea Food and Drug Administratio) ;
  • Kim, Hyo-Sun (Division of Biotechnology, Biologics Evaluation Department, Korea Food and Drug Administratio) ;
  • Shin-Won (Division of Biotechnology, Biologics Evaluation Department, Korea Food and Drug Administratio) ;
  • Lee, Seok-Ho (Division of Biotechnology, Biologics Evaluation Department, Korea Food and Drug Administratio) ;
  • Sohn, Yeo-Won (Division of Biotechnology, Biologics Evaluation Department, Korea Food and Drug Administration)
  • Published : 2002.10.01
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Abstract

A convenient bioassay of nerve growth factor(NGF) is essential for assessing its potency during the course of product development and quality controls afterwards. We have set up a cell-based bioassay for determining the potency of recombinant NGF using rat pheochromocytoma (PC12) cells. Cell survival was measured by monitoring the reduction of the alamarBlue$^{TM}$ dye by living cells. (omitted)d)

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