Proceedings of the PSK Conference (대한약학회:학술대회논문집)

The Pharmaceutical Society of Korea (대한약학회)
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Stable expression of N-terminal 3X-FLAG tagged human 5a-reductase type II in 293 cells: a new tool for protein purification ＆ inhibitor screening

  • Lee, Chang-Hoon (Skin Research Team, Skin Research Institute, Pacific R＆D Center) ;
  • Park, Won-Seok (Skin Research Team, Skin Research Institute, Pacific R＆D Cente) ;
  • An, Su-Mi (Skin Research Team, Skin Research Institute, Pacific R＆D Cente) ;
  • Nam, Gae-Won (Skin Research Team, Skin Research Institute, Pacific R＆D Cente) ;
  • Kim, Kwang-Mi (Skin Research Team, Skin Research Institute, Pacific R＆D Cente) ;
  • Kim, Seung-Hoon (Skin Research Team, Skin Research Institute, Pacific R＆D Cente) ;
  • Lee, Byeong-Gon (Skin Research Team, Skin Research Institute, Pacific R＆D Cente) ;
  • Jang, Ih-Seop (Skin Research Team, Skin Research Institute, Pacific R＆D Center)
  • Published : 2002.10.01
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Abstract

Human 5-reductase type II(5AR2) is an important target for the treatment of benign prostatic hyperplasia. In this study we describe the establishment of cell line which stably expressed 3X FLAG tagged human 5AR2. We used this cell line as a cell based assay tool and source for 5AR2 enzyme. First a plasmid (3XFLAGpCMVl0-5AR2) for the expression of 5AR2 was constructed by the use of the vector 3XFLAGpCMV10 and transfected into the HEK 293. By selection with G418 sulfate. ten HEK 293 single cell clones were obtained of which three stably exhibited high 5AR2 activity. (omitted)

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