Proceedings of the PSK Conference (대한약학회:학술대회논문집)

The Pharmaceutical Society of Korea (대한약학회)
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Purification and Characterization of Dermatan Sulfate from Eel Skin. Anguilla japonica

  • Lee, In-Seon (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Sakai-Shinobu (Department of Bioanalytical Chemistry, Graduate School of Pharmaceutical Sciences Chiba University) ;
  • Kim, Wan-Seok (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Nakamura-Ayako (Department of Bioanalytical Chemistry, Graduate School of Pharmaceutical Sciences Chiba University) ;
  • Imanari-Toshio (Department of Bioanalytical Chemistry, Graduate School of Pharmaceutical Sciences Chiba University) ;
  • Toida-Toshihiko (Department of Bioanalytical Chemistry, Graduate School of Pharmaceutical Sciences Chiba University) ;
  • Kim, Yeong-Shik (Natural Products Research Institute, College of Pharmacy, Seoul National University)
  • Published : 2002.10.01
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Abstract

Dermatan sulfate (DS) was isolated from eel skin (Anguilla japonica) bv actinase and endonuclease digeslions followed by ${\beta}$-elimination reaction and DEAE-Sephacel chromatography. DS was a major glycosaminoglycan in eel skin with 88% of the total uronic acid. The content of IdoA2S$\alpha$1longrightarrow4GalNAc4S sequence in eel skin. which is known to be a binding site to heparin cofactor II. was two times higher than that of dermatan sulfate from porcine skin. The anti-lla activity of eel skin dermatan sulfate mediated through heparin cofactor ll(NCL) was 25 units/mg. whereas DS from porcine skin shows 23.2 units/mg. The average molecular weight was determined as 14 kDa by gel chromatography on a TSKgel G3000SWXL column. Based on H1 NMR spectroscopy. we suggest that 3-sulfated and/or 2.3-sulfated ldoA residues are present in the chain.

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