대한약학회:학술대회논문집 (Proceedings of the PSK Conference)
- 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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- Pages.277.1-277.1
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- 2002
non-viral gene delivery mediated by chitosan and PEl: developement of a gene carrier with serum stability and reduced cytotoxicity
- Kim, Mi-Na (Korea Research Institute of Bioscience and Biotechnology) ;
- Hyun, Min-Sang (Korea Research Institute of Bioscience and Biotechnolog) ;
- Jiang, Ge (Korea Research Institute of Bioscience and Biotechnolog) ;
- Chung, Kyeong-Soo (Korea Research Institute of Bioscience and Biotechnolog) ;
- Yeom, Young-Il (Korea Research Institute of Bioscience and Biotechnology)
- 발행 : 2002.10.01
초록
The purpose of this study was to develop PEl-based gene carriers with optimal serum stability and reduced cytotoxicity. PEl is an efficient gene transfer agent with the ability of DNA condensation and endosome escape: however; use of the polymer in vivo is hampered by signigicant reduction in transfection activity by the presence of serum. Chitosan is a non-toxic. biodegradable and biocompatible polymer with hydrophilic functional groups so it may provide a physical stability against challenge by serum proteins. (omitted)
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