Proceedings of the PSK Conference (대한약학회:학술대회논문집)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

non-viral gene delivery mediated by chitosan and PEl: developement of a gene carrier with serum stability and reduced cytotoxicity

  • Kim, Mi-Na (Korea Research Institute of Bioscience and Biotechnology) ;
  • Hyun, Min-Sang (Korea Research Institute of Bioscience and Biotechnolog) ;
  • Jiang, Ge (Korea Research Institute of Bioscience and Biotechnolog) ;
  • Chung, Kyeong-Soo (Korea Research Institute of Bioscience and Biotechnolog) ;
  • Yeom, Young-Il (Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2002.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to develop PEl-based gene carriers with optimal serum stability and reduced cytotoxicity. PEl is an efficient gene transfer agent with the ability of DNA condensation and endosome escape: however; use of the polymer in vivo is hampered by signigicant reduction in transfection activity by the presence of serum. Chitosan is a non-toxic. biodegradable and biocompatible polymer with hydrophilic functional groups so it may provide a physical stability against challenge by serum proteins. (omitted)

Keywords