한국동물번식학회:학술대회논문집 (Proceedings of the KSAR Conference)
- 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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- Pages.48-48
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- 2002
Development of Chimeric Embryos Aggregated with Blastomeres of In Vitro Fertilization (IVF) and of Parthenote Bovine Embryos
- Yea, Eun-Ha (College of Veterinary Medicine, Gyeongsang National University) ;
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Choe, Sang-Yong
(College of Veterinary Medicine, Gyeongsang National University)
- 발행 : 2002.06.01
초록
Chimerism has become an important tool for investigating fundamental aspects of early embryonic development and differentiation in mammals for producing transgenic animals. The objective of this study was to evaluate the developmental capacity of chimeric embryos reconstructed with parthenotes and IVF bovine embryos into empty zona pellucida. The MII oocytes were activated by two treatment groups [Group 1, 5 μM inomycin, 5min, ＋ 10 ㎍/㎖ cycloheximide (CHX)/5 ㎍/㎖ cytochalasin B (CCB), 3 h; Group 2, 5 μM ionomycin, 5 min ＋ 1.9 mM 6-dimetylaminopurine (6-DMAP), 3 h]. (omitted)