Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2002.06a
- /
- Pages.41-41
- /
- 2002
Development of Bovine Embryos Reconstructed by Microinjection of Cultured Fetal Fibroblast Cells into In-Vitro Matured Oocytes
- Kim, Sungmin (College of Vet. Med., Chungnam National University) ;
- Kim, Sangkeun (College of Vet. Med., Chungnam National University)
- Published : 2002.06.01
Abstract
Bovine cumulus-oocyte complexes were recovered from ovaries at a slaughter and then divided into five groups: control group(unvitrified oocytes), 0 hr. group(composed of oocytes vitrified before the onset of maturation) and 10, 14, and 20 hrs groups(vitrified respectively at 10, 14 and 20 hrs after the onset of maturation). The oocytes remained vitrified for 24 hrs, and then were thawed in 30℃ water bath. Survival and cleavage rates were defined as development rate on in vitro culture and stained with aceto-orcein or FDA test.