Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2002.06a
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- Pages.36-36
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- 2002
Interspecies Nuclear Transfer using Bovine Oocytes Cytoplasm and Somatic Cell Nuclei from Various Mammalian Species
- Park Se-Yeong ;
- Kim Eun-Yeong ;
- Yun Ji-Yeon ;
- Gil Gwang-Su ;
- Kim Seon-Gyun ;
- Lee Chang-Hyeon ;
- Park Se-Pil ;
- Im Jin-Ho
- Published : 2002.06.01
Abstract
This study was designed to examine the ability of the bovine (MII) oocytes cytoplasm to support several mitotic cell cycles under the direction of differentiated somatic cell nuclei of bovine, human, porcine and mouse. Bovine GV oocytes were matured in TCM-l99 supplemented with l0% FBS. At 22 h after IVM, denuded recipient oocytes were stained with 5 ㎍/㎖ Hoechst and their 1 st polar body (PB) and MII plate were removed by enucleation micropipette under. (omitted)