Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2002.06a
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- Pages.23-23
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- 2002
Effect of Sucrose and Polybrene on the Gene Transfer into Procine Oocytes using Retroviral Vector
- Kim, Kang-Sig (Animal Resource Research Center, Konkuk University) ;
- Kim, Teoan (Animal Resource Research Center, Konkuk University) ;
- Ju, Jin-Young (Animal Resource Research Center, Konkuk University) ;
- Kim, Kwang-Sung (Animal Resource Research Center, Konkuk University) ;
- Lee, Hoon-Taek (Animal Resource Research Center, Konkuk University) ;
- Chung, Kil-Saeng (Animal Resource Research Center, Konkuk University)
- Published : 2002.06.01
Abstract
In vitro matured porcine oocytes have very small volume of perivitellinespace(PVS). In these respect, the effects of sucrose and polybrene on the efficiency of gene transfer were investigated. As a gene (hGH) transfer vehicle, Vesicular stomatitis virus glycoprotein pseudotyped retroviral vector (VSV-G) was used. Sucrose treatment have no detrimental effect on the rates of cleavage and following development and induced the enlargement of PVS resulting the efficient introduction of retroviral vector stocks into PVS. (omitted)