Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2002.06a
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- Pages.9-9
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- 2002
In Vitro Development of Somatic Cell Nuclear Transferred Bovine Embryos Following Activation Timing in Mil Enucleated Oocytes Cryopreservation
- Kim Seon-Gyun ;
- Kim Eun-Yeong ;
- Gil Gwang-S ;
- Park Se-Yeong ;
- Yun Ji-Yeon ;
- Park Se-Pil ;
- Jeong Gil-Saeng ;
- Im Jin-Ho
- Published : 2002.06.01
Abstract
This study was to evaluate the in vitro survival of vitrified-thawed bovine MII enucleated (MIIe) oocytes according to activation timing and minimun volume cooling (MVC) method and their in vitro development after somatic cell nuclear transfer (SONT). Bovine oocytes were recovered from slaughtered bovine ovary and matured in TCM-199 supplemented with 10% FBS. (omitted)