한국수정란이식학회:학술대회논문집 (Proceedings of the Korean Society of Embryo Transfer Conference)
- 한국수정란이식학회 2002년도 국제심포지엄
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- Pages.116-116
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- 2002
Expression of Cyclin B1 mRNA and Protein after Activation in Enucleated Mouse Oocytes
- Hwang, Seong-Soo (Samsung Medical Center, Chung-Ang University) ;
- Kim, Chang-Kun (Samsung Medical Center, Chung-Ang University) ;
- Chung, Young-Chai (Samsung Medical Center, Chung-Ang University)
- 발행 : 2002.11.01
초록
Further development of reconstructed embryos may be dependent upon the synchronization of donor nucleus and recipient cytoplasm at cell fusion, To control the synchronization of donor and recipient cells, the enucleated MII arrested oocytes are artificially stimulated prior to embryo reconstruction. Destruction of cyclin B results in the exit of cells from M-phase of cell cycle. This study was designed to investigate the effects of single or combined stimulation affected cyclin B1 mRNA and protein levels in mouse oocytes. The oocyte activation was induced by 7% ethanol or 10