한국수정란이식학회:학술대회논문집 (Proceedings of the Korean Society of Embryo Transfer Conference)
- 한국수정란이식학회 2002년도 국제심포지엄
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- Pages.90-90
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- 2002
Effect of Sugar Combination in Tris-buffer on the Viability of Post-thaw Spermatozoa in Canine
- Yu, D.J. (Department of Animal Science and Technology, Sunchon National University) ;
- Jeong, S.R. (Department of Animal Science and Technology, Sunchon National University) ;
- Oh, I.S. (Department of Animal Science and Technology, Sunchon National University) ;
- Bae, I.H. (Department of Animal Science and Technology, Sunchon National University) ;
- Cho, S.G. (Department of Animal Science and Technology, Sunchon National University) ;
- Kong, I.K. (Department of Animal Science and Technology, Sunchon National University)
- 발행 : 2002.11.01
초록
The purpose of this study was to investigate the effect of kind and combination of sugars on the viability and acrosome damage of post-thaw spermatozoa in canine. The extender used was Tris-citric acid extender (Tris-buffer) supplemented with 20% Egg-yolk, 8% glycerol, 1% Equex STM paste, and 70 mM sugars such as monosaccharide (fructose and xylose) and disaccharide(trehalose). To evaluate of sugar combination, the sugars supplemented in Tris-buffer were combined such as single (fructose, xylose, trehalose), two combinations (Fruc+Tre, Fruc+xyl, Tre+xyl) and three combinations (Fruc+Tre+Xyl). The concentration of sperm collected were adjusted of 50