금속가공유 시료에서 Optical Density 설정조건에 따른 엔도톡신의 정량

Analysis on Endotoxin Using Analytical Conditions of Optical Density in Metalworking Fluid Sample

This study was conducted to identify the proper analytical conditions regarding optical density for endotoxin analysis in the workplace using metalworking fludis. Our study found the "onset method" was more accurate than "time to Vmax" Reproducibility and accuracy analyzed by "onset method" was more greater than those by "time to Vmax""0.03"of optical density was the most appropriate analytical condition among onset method. Through this analytical condition, 0.998 of linearity was obtained and recovery rate ranged from 88％ to 105％ at endotoxin concentration below 5 EU/$m\ell$. No significant difference of endotoxins between "0.03"and "0.05" of optical density was observed from this study. Furthermore, correlation coefficients between them were statistically significant(p＜0.01). This study concluded that 0.03”or "0.05" of optical density is used to analyze endotoxin. Of these optical density value, value with higher linearity should be used to analyze endotoxin in environmental samples.