한국어업기술학회:학술대회논문집 (Proceedings of the Korean Society of Fisheries Technology Conference)
- 한국어업기술학회 2002년도 춘계 수산관련학회 공둥학술발표회
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- Pages.279-280
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- 2002
Genetic Similarity and Difference of Marsh Clam (Corbicula leana) Obtained by RAPD-PCR
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Yoon, Jong-Man
(Department of Marine Biomedical Science, Kunsan National University) ;
- Park, Hwan-ha (Department of Marine Biomedical Science, Kunsan National University) ;
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Choe, Sun-Nam
(Department of Food Science & Technology, Kunsan National University)
- 발행 : 2002.05.01
초록
Genomic DNA from the muscle of marsh clam (Corbicula leana) from Gochang was extracted in order to identify genetic differences and similarity by randomly amplified polymorphic DNAs-polymerase chain reaction. 3.28 of the 23.0 polymorphic bands per lane were found to be polymorphic in marsh clam. Also, about 4.34% of total polymorphic bands were either specific to marsh clam. The major common bands of 0.28 kb generated by primer OPB-15 (GGAGGGTGTT) were present in every individuals, respectively, which were polymorphic. This common bands which present in every individuals should be diagnostic of specific strains, species and/or their relatedness. Primer OPB-19 (ACCCCCGAAG) produced the highest number of specific bands, which was 12. The specific minor band of 0.07 kb was present in lane 22, which were polymorphic. Especially, only a specific band (1.35 kb) identifying individuals was observed in lane 22.
키워드