한국생물공학회:학술대회논문집
- 2001.11a
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- Pages.779-781
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- 2001
Saccharomyces cerevisiae에서의 비만 억제용 재조합 단백질 leptin 생산 연구
- Published : 2001.11.07
Abstract
Human leptin is a 16kDa protein and is known to influence body weight control. It is composed of 146 amino acids. In this study, human leptin gene was obtained from homosapiens leptin mRNA using RT-PCR. And leptin gene was inserted into secretion vectors and Saccharomyces cerevisiae was transformed. In flask culture, Saccharomyces cerevisiae transformant showing high leptin expression titre was selected and with the best transformant, fed-batch fermentation and purification was optimized. As a result, about 2 g/L of leptin was expressed and the yield of purification was about 80%.
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