한국생물공학회:학술대회논문집
- 2001.11a
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- Pages.767-770
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- 2001
Enhancement of human serum albumin production in Saccharomyces cerevisiae
- Seo, Dong-Sik (Division of Environmental and Biological Engineering, Kangwon National University) ;
- Choi, Byung-Hyun (Division of Environmental and Biological Engineering, Kangwon National University) ;
- Kang, Whan-Koo (Department of Chemical Engineering, Hannam University) ;
- Kim, In-Ho (Department of Chemical Engineering, Chungnam National University) ;
- Hong, Eock-Kee (Division of Environmental and Biological Engineering, Kangwon National University)
- Published : 2001.11.07
Abstract
Experiments were accomplished to reduce the extent of proteolysis by simply controlling the culture conditions instead of the gene manipulation techniques. L-arginine and L-lysine were chosen as a protease inhibitor analogue With the assumption that they might act as the potential inhibitors against proteases involved in the rHSA proteolysis. The addition of arginine and lysine resulted in a considerable positive effect on the secreted rHSA production level.
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