한국생물공학회:학술대회논문집
- 한국생물공학회 2001년도 추계학술발표대회
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- Pages.754-757
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- 2001
Cell growth and GFP expression in E. coli BL21 and W3110 under coexpression of Vitreoscilla hemogobin
초록
Expression of the vhb gene encoding bacterial hemoglobin (VHb) from Vitreoscilla has been used to improve recombinant cell growth and enhance product formation under microaerobic conditions because of its ability to enhance oxygen use. We coexpressed GFP and VHb in Escherichia coli BL21 and W3110, and compared with GFP control which was not expressed VHb. We used nar oxygen-dependent inducible promoter for VHb expression. The GFP amounts in E. coli expressed VHb was about five fold higher than in the control Fluorescence intensity was increased about two fold.
키워드