P. aeruginosa EMS1의 mutagen 처리를 통한 고기능 유화재 균주의 개발

생물 계면활성제의 개발을 위해 MNNG(N-Methyl-N-Nitro- NitrMoguanidjne), EMS, UV radiator 등 random mutation을 유도하여 가장 고기능 유화제 생산 균주를 선별하여 다양한 탄소원에 따른 생육도와 유화활성, 유화 활성에 미치는 pH 등 생물 계면활성제의 생산에 관한 조사를 한 결과 Bunker A에서는 유화활성이 원래 균주보다 최고 약 6배까지 증가했으며 표면장력 또한 40.3dyne/cm 에서 34.0dyne/cm 으로 크게 감소되었다.

This study was performed to improve the efficency of production of biosudactant which were produced by newly screened MNNGCN-Methyl-N-Nitro- Nitrosoguanidine) mutagenized P. aeruginosa EMS1. A culture grown exponentially for $30^{\circ}C$ in trypic soy brotb is adjusted to pH. MNNG is added and incubated in water bath shaker at about 250 ${\sim}$300rpm. After 20 min, is dilutecl into colded trypic soy broth and centrifugation. The cell pellet is resuspended in 50$m{\ell}$ of trypic soy broth. Cultures are grown at $30^{\circ}C$ overnight. cetyltrimethylammonium bromide-metbylene blue agar plate selected dark blue halo colony. Peanut oil, Castor oil, Olive oil, and so on were compared as carbon source of surface tension and emulsifying activity.