한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effect of Media Compositions on Mycelial Growth of L. edodes

  • 박원순 (강원대학교 환경 생물공학부) ;
  • 지영민 (고려대학교 생명 유전공학부) ;
  • 최정우 (서강대학교 화학공학과) ;
  • 홍억기 (강원대학교 환경 생물공학부)
  • Published : 2001.11.07
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Abstract

This study was to investigate the effects of media components on the mycelial growth of Lentinus eclodes, including C-source. N-source, Inorganic salts, and c/N ratio. Glucose and yeast extract were selected as C-source and N-source, respectively. $KH_2PO_4,\;K_2HPO_4,\;MGSO_4,\;7H_2O$ as inorganic salts were added. When glucose concentration was 30g/L and yeast extract concentration was 20g/L, indicating that C/N ratio was 1.5, the cell mass was about 9g/L.

본 연구는 Lentinus edodes의 액체배양을 통하여 물리 ${\cdot}$ 화학적인 방법을 이용, 고농도의 탄수화물 복합효소를 얻어 hemicellulose와의 반응을 거쳐 고수율의 아라비 녹실란을 얻는데 그 목적이 있다. 온도 $24^{\circ}C$, pH 5.5. working volume 100mL, 접종비 5%. 200rpm이며 가장 적합한 C-source 로서는 glucose가 선택되었으며 N - source로서는 yeast extract가 선택되었다.

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