PCR detection of food-borne pathogenic microorganisms in milk

Milk is easily contaminated by pathogenic microorganisms and contains many ingredients that inhibit normal PCR. In this study, we developed a detection mothed for pathogenic microorganisms existing in milk by usting PCR. 'Sample pretreatment prior to PCR were compared to overcome the inhibition. A high PCR efficiency was achieved by SDS lysis pretreatment. without further purification of DNA for PCR.

본 실험에서는 우유에 존재하는 특정미생물을 PCR 에 의해 검출하기 위한 시료 전처리 방법을 비교 하였다 . PCR 혼합물 내에 우유의 함량이 높아지면 PCR이 저해됨을 알 수 있으며 SDS lysis 처리를 한 후의 PCR 효율이 가장 좋았다.