한국생물공학회:학술대회논문집
- 2001.11a
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- Pages.185-188
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- 2001
Biosynthesis of Carbohydrate associated with secondary Metabolites; Biosynthesis of dTDP-4-keto-6-deoxy-D-glucose by four enzyme reaction system
Abstract
We have developed a synthetic method for dTDP-4-keto-6-deoxy-D-glucose with four enzyme system. We have used crude extracts from cultures of Escherichia coli BL21 strains harboring plasmids containing different sources. dTDP-4-keto-6-deoxy-D-glucose was synthesized by the combination of thymidine-monophosphate kinase, acetate kinase, dTDP-glucose synthase and dTDP-D-glucose 4,6-dehydratase in a batch system, starting the reaction with dTMP. The enzymatic synthesis strategy allowed a dTMP conversion with a 95%.
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