Proceedings of the Korean Society for Applied Microbiology Conference (한국미생물생명공학회:학술대회논문집)
- 2001.06a
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- Pages.141-145
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- 2001
Metabolic Engineering of Nonmevalonate Pathway in Escherichia coli Enhances Lycopene Production
- Kim, Seon-Won (Dept. of Food Science and Nutrition Gyoungsang National University) ;
- J.D. Keasling (Dept. of Chemical Engineering UC Berkeley)
- Published : 2001.06.01
Abstract
Isopentenyl diphosphate (IPP) is the common, five-carbon building block in the biosynthesis of all carotenoids. IPP in Escherichia coli is synthesized through the non-mevalonate pathway. The first reaction of IPP biosynthesis in E. coli is the formation of l-deoxy-D-xylulose-5-phosphate (DXP), catalyzed by DXP synthase and encoded by dxs. The second reaction in the pathway is the reduction of DXP to 2-C-methyl-D-erythritol-4-phosphate, catalyzed by DXP reductoisomerase and encoded by dxr. To determine if one or more of the reactions in the non-mevalonate pathway controlled flux to IPP, dxs and dxr were placed on several expression vectors under the control of three different promoters and transformed into three E. coli strains (DH5
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