한국미생물생명공학회:학술대회논문집 (Proceedings of the Korean Society for Applied Microbiology Conference)
- 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
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- Pages.62-72
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- 2001
Chaperone Assisted Overexpression of D-carbamoylase Independent of the Redox State of Host Cytoplasm
- Sareen, Dipti (Institute of Microbial Technology) ;
- Sharma, Rakesh (Institute of Microbial Technology) ;
- Vohra, Rakesh M. (Institute of Microbial Technology)
- 발행 : 2001.06.01
초록
The N-carbamoyl-D-amino acid amidohydrolase (D-carbamoylase) gene (dcb) from Agrobacterium tumefaciens AM 10 has been successfully cloned and expressed in Escherichia coli. Expression of D-carbamoylase gene under the 17 promoter in different host strains showed that the optimal expression was achieved in E. coli JM109 (DE3) with a 9-fold increase in enzyme production compared to the wild-type strain. The co-expression of the GroEL/ES protein with D-carbamoylase protein caused an in vivo solubilization of D-carbamoylase in an active form. The synergistic effect of GroEL/ES at 28
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