RPA-governed Endonuclease Switching during Eukaryotic Okazaki Fragment Processing.

  • Bae, Sung-Ho (National Creative Research Initiative Center for Cell Cycle Control, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine) ;
  • Bae, Kwang-Hee (National Creative Research Initiative Center for Cell Cycle Control, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicin) ;
  • Kim, Jung-Ae (National Creative Research Initiative Center for Cell Cycle Control, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicin) ;
  • Seo, Yeon-Soo (National Creative Research Initiative Center for Cell Cycle Control, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine)
  • 발행 : 2001.06.01

초록

At the eukaryotic replication fork, discontinuous synthesis of lagging strand DNA gives rise to Okazaki fragments carrying ribonucleotides derived from the primer RNA at their 5' ends. Efficient removal of these ribonucleotides is vital for maintaining genome integrity. In this report we show that the endonucleases Dna2 and Fen1 act sequentially to facilitate the complete removal of the primer RNA.(omitted)

키워드