Proceedings of the Korean Society of Toxicology Conference (한국독성학회:학술대회논문집)

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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SYNERGISTIC EFFECT OF HUMAN CYTOCHROME B5 COEXPRESSION ON THE METABOLIC ACTIVITY OF CYP1A2 IN CHINESE HAMSTER OVARY CELLS

  • Kang, Jin-Sun (College of Medicine and Institute of Biomedical Sciences, Hanyang University) ;
  • Kang, Hyuck-Joon (College of Medicine and Institute of Biomedical Sciences, Hanyang University) ;
  • Dong, Mi-Sook (Graduate School of Biotechnology, Korea University) ;
  • Park, Chang-Hwan (College of Medicine and Institute of Biomedical Sciences, Hanyang University)
  • Published : 2001.10.01
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Abstract

Human cytochrome B5 (CYB5) was coexpressed with cytochrome P450 1A2 (CYP1A2), NADPH-CYP450 reductase (CYPR) and Ν-acetyltransferase 2 (NAT2) in Chinese hamster ovary (CHO) cells. The expression of four proteins was determined by Western blot analyses. The introduction of cDNAs to CHO cells were transduced via retroviral vectors. The cytotoxicity assay of 2-aminoanthracene (2-AA) and aflatoxin B$_1$were approximately 4-fold more sensitive than CYB5 free cells.(omitted)

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