Proceedings of the Korean Society of Toxicology Conference (한국독성학회:학술대회논문집)

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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STABILIZATION OF CYP3A4 mRNA BY CO-EXPRESSION OF CYTOCHROME $B_5$ IN E. COLI.

  • Kim, Hyun-Jung (Graduate school of Lifescience and Biotechnology, Korea University) ;
  • Park, Young-In (Graduate school of Lifescience and Biotechnology, Korea University) ;
  • Dong, Mi-Sook (Graduate school of Lifescience and Biotechnology, Korea University)
  • Published : 2001.05.01
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Abstract

Human cytochrome P450 (CYP or P450) 3A4 (CYP3A4) is the most abundant among P450s in human liver. We previously reported that the expression of CYP3A4 in membranes prepared from E. coli coexpressed the bicistronic construct of CYP3A4 and NADPH-P450 reductase with cytochrome b$_{*}$ (b5) was showed 20-60% higher than that in membranes from E. coli expressed only the bicistronic construct with culturing longer times (48-72h).(omitted)

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