Scanning Electron Microscope Observations in the Apices of Roots with Refractory Apical Periodontitis.

  • Matsuo, T. (Department of Conservative Dentistry, The University of Tokushima School of Dentistry) ;
  • Fujinaka, K. (Department of Conservative Dentistry, The University of Tokushima School of Dentistry) ;
  • Yoshida, Y. (Department of Conservative Dentistry, The University of Tokushima School of Dentistry) ;
  • Ozaki, K. (Department of Conservative Dentistry, The University of Tokushima School of Dentistry)
  • 발행 : 2001.11.01

초록

The aim of this study was to examine the localization of bacteria in the apices of roots with refractory apical periodontitis by a scanning electron micrscopy(SEM), and to identify the extra-radical bacteria using a immunohistological method. Eleven teeth were prepare for immunostaining and 4 for SEM. Immunostaining was performed with LSAB method and specific antisera against 18 bacteria selected for this study. Specimens for SEM observations were dried using a t-butyl alcohol freeze dryer and coated with gold-palladium.(omitted)

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