한국동물번식학회:학술대회논문집 (Proceedings of the KSAR Conference)

한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Changes in Acrosin Activity and Membrane Function of Boar Spermatozoa

  • Kim, C.K. (Department of Animal Science and Technology, Chung-Ang University) ;
  • Kim, I. (Department of Animal Science and Technology, Chung-Ang University) ;
  • Y.C. Chung (Department of Animal Science and Technology, Chung-Ang University) ;
  • J.W. Ryu (Department of Animal Science and Technology, Chung-Ang University) ;
  • H.J. Yoon (AI Center, Darby Pig Breeding Co.) ;
  • K. Kang (AI Center, Darby Pig Breeding Co.) ;
  • Kim, I.C. (National Livestock Research Institute) ;
  • Lee, J.H. (National Livestock Research Institute) ;
  • S.E. Yeon (Department of Animal Science and Technology, Chung-Ang University)
  • 발행 : 2001.03.01
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초록

The aims of this work were to determine the acrosin activity and to evaluate the structural and functional integrity of AS boar spermatozoa. The acrosin activity of spermatozoa were 5.40, 4.10 and 3.40 mIU/10$^{6}$ sperm in raw, extended and frozen semen respectively , which differed significantly each other (P<0.05). After the raw and extended semen were exposured to cold and thermal shock, the acrosin activities of spermatozoa in the raw semen were 5.39, 5.21 and 5.29 mIU/10$^{6}$ sperm for control (non-shock), cold shock and thermal shock, and those of extended semen were 4.21, 3.98 and 4.00 mIU/10$^{6}$ sperm. This value among treatments did not differ significantly. The acrosin activities of spermatozoa in the extended and stored semen were 3.27, 3.52, 3.46 and 3.23 mIU/10/suup 6/ sperm, while hypo-osmotic test(HOST) values were 56.5%, 64.7%, 66.0% and 56.0%, following 4 days storage at 4$^{\circ}C$, 17$^{\circ}C$ , $25^{\circ}C$ and 37$^{\circ}C$, respectively. The results at 17$^{\circ}C$ and $25^{\circ}C$ appeared to be best compared with the other storage temperatures.

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