한국동물번식학회:학술대회논문집 (Proceedings of the KSAR Conference)
- 한국동물번식학회 2001년도 춘계학술발표대회
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- Pages.68-68
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- 2001
Morphological Characteristics of Pig Blastocysts Produced by Somatic Cell Nuclear Transfer
- Y.M. Han (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- D.B. Koo (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- Park, Y.H. (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- Park, J.S. (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- Kim, H.N. (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- Y.K. Kang (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- W.K. Chang (National Livestock Research Institute) ;
- Lee, K.K. (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology)
- 발행 : 2001.03.01
초록
Blastocyst formation, consisting of the inner cell mass (ICM) and trophectoderm (TE), is the first differentiation process during embryonic development in mammals. It has been hypothesized that the proportion of ICM to TE in the blastocyst may be crucial for subsequent developmental competence of early embryos, which it may be expressed as a sensitive indicator for evaluating in vitro systems. In this study ICM/total cell ratio of nuclear transfer (NT) embryos was compared with IVF-derived and in vivo embryos. Somatic cell nuclei obtained from a fetus at Day 40 of gestation were transferred into the enucleated oocyte and then cultured in NCSU 23 medium for 6 days as previously described (Koo et al., Biol. Reprod. 2000; 63:986-992). ICM and TE cells of blastocysts were determined by using a differential staining method (Han et al., Biol. Reprod. 1999; 60:1110-1113). Development rate (9.8