A Study on the Analysis of Gel Images of Genes and Molecules

유전자 및 물질의 젤 영상 분석에 관한 연구

With all the researches to define human genom and to look for some new bio-activated material in the bio-technology field recently, it is more highly needed to analyse DNA or so called Material than ever before. First, the lanes are extracted based on histogram analysis and projection technique. And then three other approaches are applied for band extraction: SB, RG-1, and RG-2. In SB method, a search line is set dividing each lane equally and vertically to find peaks and valleys. And according to them, minimum enclosing rectangle of each band is determined. In RC-1 approach, on the other hand, band areas are extracted by region growing with the peaks as seeds, avoiding the overlap with the neighboring bands. In RC-2 approach, peaks and valleys are searched in two lines that trisect the lane vertically, and the pair of peaks in the same band are determined, and then used to grow the region. To compare the accuracy of the three suggested methods, we measure the location and amount of bands. The result shows that the mean deviation of the location is 0.06, 0.03, and 0.01 for SB, RG-1, and RC-2 respectively. And the mean deviation of the amount of bands is 0.08, 0.05, and 0.02 for SB, RG-1, and RG-2 respectively. In conclusion, the RG-2 method suggested in this paper appears to be the most reliable on the degree of the accuracy in measuring the location and amount of bands