한국발생생물학회:학술대회논문집 (Proceedings of the Korean Society of Developmental Biology Conference)
- 한국발생생물학회 2001년도 전기 제11차 학술대회 논문집
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- Pages.55-56
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- 2001
Bovine Follicular fluid and Serum Share a Unique Isoform of Matrix Metalloproteinase-2 That Is Degraded by the Oviductal Fluid
- Kim, Minjung (Laboratory of Developmental Biology, Seoul Womens Univ.) ;
- Minjeong Hong (Laboratory of Developmental Biology, Seoul Womens Univ.) ;
- Kim, Jisoo (Laboratory of Developmental Biology, Seoul Womens Univ.) ;
- Kim, Haekwon (Laboratory of Developmental Biology, Seoul Womens Univ.) ;
- Lee, Seung-Jae (Mirae & Heemang Ob/Gyn Clinic) ;
- Kang, Sung-Goo (Department of Biology, College of Natural Science, Inje Univ.) ;
- Cho, Dong-Jae (Department of Ob/Gyn. College of Medicine, Younsei Univ.)
- 발행 : 2001.02.01
초록
Gelatin zymograms of bFF and bS showed GA110 and 62 kDa gelatinses in adsition to several minor ones. Of these, GA110 gelatinase was abolished by treating bFF or bS with bOF and interestingly, its enzymatic activity was enhanced by adding EDTA to bFF or bS before zymographic analyses. Experiments using specific inhibitors of MMPs indicated that GA110 and 62 kDa proteins were indeed gelatinases. Immunoblotting experiments using an antibody against human MMP-2 showed that both GA110 and 62 kDa were an MMP-2 isoform and active MMP-2, respectively. The results suggest that the interaction between bFF and bOF can occur at the time of fertilization.
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