Multi-Channel Two-Stage 시스템을 이용한 수질 독성 모니터링의 지표 확립 및 모사

The character of a recombinant bioluminescent bacteria's light emission enables us to monitor toxicity in water, soil and air. In this study, various bioluminescent responses to water samples containing toxic chemicals, such as phenol and mitomycin C, were obtained and analysed through the use of a multi-channel two-stage minibioreactor system. The bioluminescent pattern from each channel can be used as a standard for identifying the degree of toxicity in field samples. When various concentrations of toxic chemicals were injected in a step manner, different bioluminescent patterns were obtained. Also this system showed variation in its bioluminescent pattern as the injection manner was changed, i.e. using a modified version of the bell-curve type injection. In conclusion, the toxicity was shown to be related with the bioluminescent response when using these standard bioluminescent patterns. Comparing this standard with a bioluminescent response from a field sample, we can estimate the degree of which the sample is toxic.

본 실험을 통하여 각 채널에서의 독성 농도에 따른 다양한 발광 패턴을 확보 할 수 있었으며 이를 바탕으로 본 시스템의 실제 현장 적용 시 나타나는 발광 패턴과 비교하여 독성을 분석하고 정성, 정량화 하는데 지표로 사용될 수 있다.