Acceptor reaction of a novel transfructosylating enzyme from Bacillus sp.

Many different oligosaccharides were produced by transferring the fructose residue of sucrose to maltose, cellobiose, lactose and sucrose (self-transfer), where their yields of fructosylated acceptor products accounted for $26{\sim}30%$ (w/w). The maximum conversion yield (30%) was obtained in fructosyl cellobioside formation with 500 g sucrose/l (substrate) and 200 g cellobiose/l (acceptor). These four acceptors gave various products having DP (degree of polymerization) $2{\sim}7$ by successive transfer reactions.