Abstract
Characteristics of $CO_2$ fixation by Chlorella sp. HA-1 were studied in a semi-continuous mode in a series operation. A high $CO_2$ fixation rate for a long time was obtained, when the method of semi-continuous mode was employed, in which the controlling parameter was the dilution ratio. A constant $CO_2$ fixation rate was maintained even when the dilution ratio was increased with the increment of 0.1 at the initial value of 0.5. The method of series operation was used to improve the efficiency of $CO_2$ fixation. The total amount of $CO_2$ fixation was proportion to the number of reactor in series operation.