미생물을 이용한 원유 및 원유제품의 분해 특성

Crude oil-degrading microorganisms, Acinetobacter sp. A132, Pseudomonas aeruginosa F722, and Acinetobacter calcoaceticus OM1 were isolated from soil and sea. The optimal temperature of strain A132 and strain F722 on growth isolated from soil was $35^{\circ}C$ both, and also their growth were optimized at pH 8 and 9, respectively. The growth of the strains, A132 and F722, showed that crude oil of 2% (w/v) in culture broth in which crude oil was used as carbon and energy sources appeared to be an optimum. Optimal culture conditions of strain OM1 were different from those of the soil microorganisms except for temperature. The growth of strain OM1 was optimized at pH 7 and crude oil of 3.0% (w/v). The degradability to crude oil by strain A132 showed maximum $5.49g/\;l\;{\cdot}\;day$ under the conditions of $25^{\circ}C$, NaCl of 1.0% (w/v), and crude oil of 2.0% (w/v). The highest degradability of strain F722 to crude oil was $1.19g/\;l\;{\cdot}\;day$ under the culture conditions at $35^{\circ}C$, NaCl 1.0% (w/v), and crude oil of 2.0% (w/v). The degradation characteristics of kerosene $(nC_9-nC_{20})$ and diesel $(nC_9-nC_{28})$ by strain OM1, and F722 were analyzed by gas chromatography. Strain OM1 degraded more than 95% of kerosene and 75% of diesel for 7 days cultivation. Strain F722 showed degradation of more than 80% to kerosene in 10 days.

토양으로부터 2종류 그리고 해양으로부터 1종류의 원유 분해 미생물을 분리하였고, 이들을 strain A132, strain F722 그리고 strain OM1으로 각각 명명하였다. 이들 미생물은 Acinetobacter sp., Pseudomonas aeruginosa, Acinetobacter calcoaceticus로 각각 동정되었다. Strain A132. F722의 최적 배양 및 분해온도는 $35^{\circ}C$ 이고, 최적 생장 pH는 각각 8과 9에서 나타났다. 원유를 유일한 탄소원으로 하여 배양을 하였을 때 원유농도 2.0%(w/v)에서 생장이 높았다. 한편, 해양에서 분리된 strain OM1은 pH 7, 원유농도 3.0%(w/v)에서 생장이 높았다. 원유 분해능 조사에서는 Eleuthera (OMAN) 원유를 2.0%(w/v) 기질로 하였을 때, strain A132가 $25^{\circ}C$에서 $5.49g/\;l\;{\cdot}\;day$의 분해능을 나타내었다. 반면, L-Zakum (AFRICA) 원유에서는 strain F722가 $35^{\circ}C$에서 $1.19g/\;l\;{\cdot}\;day$의 분해능을 보였다. 등유$(nC_9-nC_{20})$와 경유$(nC_9-nC_{28})$에 대하여 분해특성을 조사한 결과 strain OM1은 배양 7일 후 95, 75%를 각각 분해하였다. Strain F722는 배양10일 후 80%의 분해율을 나타내었다.