한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Hydrogen peroxide, its measurement and effect during enzymatic decoloring of Congo Red

  • 우성환 (인하대학교 생물공학과 생물환경소재 연구실) ;
  • 조정숙 (인하대학교 생물공학과 생물환경소재 연구실) ;
  • 김갑정 (인하대학교 생물공학과 생물환경소재 연구실) ;
  • 김은기 (인하대학교 생물공학과 생물환경소재 연구실)
  • Published : 2000.11.09
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Abstract

The color of textile-wastewater hindered spectrometric measurements of $H_2O_2$ and enzyme activity during enzymatic decoloring. By using ABTS, we developed a new method for measuring peroxidase activity and $H_2O_2$ concentration. The ratio of enzyme and $H_2O_2$ was optimized as 1:150 by investigating the effects of $H_2O_2$ on enzymatic decoloring. Pulse feeding of $H_2O_2$, upon depletion, significantly increased the decoloring of Congo Red.

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