초록
Chlorella vulgaris를 이용하여 기본배지에서 초기 주입농도 $0.7{\times}10^4\;cells/mL$ 상태에서 300시간 배양 후 $1.21{\times}10^5\;cells/mL$의 생체수를 얻었으며, 초기 cell 농도가 0.30g/L인 경우가 성장률이 $0.162g/L\;{\cdot}\;day$로 우수하였다. Glucose 초기농도가 2.00g/L인 경우 초기 cell 농도 0.025g/L에서 140시간 후 0.874g/L이 되었으며 비성장속도는 $0.243h^{-1}$을 나타내었고, 72시간내에 가스를 268mL를 생성하였으며 이후로는 수소 생성이 종료으나 순수 소소를 19.87mol% 함유하고 있었다. Chlorella vulgaris의 조성을 원소분석기로 분석한 결과 $C_{1.000}\;H_{1.774}\;N_{0.125}\;O_{0.557}$가 되었으며 이산화탄소 전환율은 $0.616cells/\;g\;CO_2$가 됨을 알 수 있었다.
After 300hours cultivation Chlorella vulgaris in standard medium in which the initial cell seeding concentration of $0.7{\times}10^4ells/mL$, $1.21{\times}10^5ells/mL$ was gained but in the case of 0.30g/L of initial cell seeding concentration the maximum growth rate of $0.162g/L\;{\cdot}\;day$ was shown. In the case of the initial glucose concentration of 2.00g/L, the cell concentration was changed from initial 0.025g cells/L to 0.874g cells/L after 140hours cultivation, the specific growth rate was $0.243h^{-1}$, but 268mL of gases were formed in 72hours, and after that, hydrogen evolution was completed. Formed gases were not all hydrogen, and 19.87 mol% of hydrogen is detected by GC. Analyzing the composition of Chlorella vulgaris by elementary analysis, it is found to be $C_{1.000}\;H_{1.774}\;N_{0.125}\;O_{0.557}$, and $CO_2$ conversion rate by Chlorella vulgaris was $0.616\;cells/g\;{\cdot}\;CO_2$