한국생물공학회:학술대회논문집
- 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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- Pages.48-51
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- 2000
In vitro Polymerization and Copolymerization of Poly-3-hydroxypropionyl-CoA with the PHB Synthase from Ralstonia eutropha
- 송재준 ;
- ;
- Song, Jae-Jun (Polymer Science and Engineering Department, University of Massachusetts, Korea Research Institute of Bioscience and Biotechnology) ;
- Goodwin, Steve (Department of Microbiology, University of Massachusetts) ;
- Lenz, Robert W. (Polymer Science and Engineering Department, University of Massachusetts)
- 발행 : 2000.11.09
초록
The poly(3-hydroxybutyrate) (PHB) synthase of Ralstonia. eutropha, which was produced by a recombinant strain E. coli and purified in one-step with a methyl-HIC column to a purity of more than 90%, was used to polymerize 3-hydroxypropionyl-CoA (3HPCoA) and to copolymerize 3HPCoA with 3-hydroxybutyryl-CoA (3HBCoA) in vitro. A
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