한국생물공학회:학술대회논문집
- 한국생물공학회 2000년도 춘계학술발표대회
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- Pages.478-481
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- 2000
재조합 효모를 이용한 endoinulinase의 생산 특성
초록
The INU2 gene encoding an endoinulinase of Aspergillus ficuum was expressed by the Kluyveromyces marxianus INU1 promoter in a SUC2-deleted Saccharomyces cerevisiae to produce the endoinulinase free of an exoinulinase and an extracellular invertase in the culture medium. When inulin was included in the medium, a recombinant yeast strain produced the sufficient amount of the enzyme to make a halo around its colony. An expression of endoinulinase was dependent on the culture temperature and shaking. The highest expression of endoinulinase was observed at
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