Optimization of C/N ratio for production of pullulan

  • Seo, Hyeong-Pil (Faculty of Natural Resources & Life Science, Dong-A University) ;
  • Kim, Hyeon-Suk (Faculty of Natural Resources & Life Science, Dong-A University) ;
  • Kim, Mi-Ryeong (Dept. of Biotechnology & Bioengineering, College of Fisheries Science, Pukyung National University) ;
  • Kim, Seong-Gu (Dept. of Biotechnology & Bioengineering, College of Fisheries Science, Pukyung National University) ;
  • Lee, Jin-U (Faculty of Natural Resources & Life Science, Dong-A University)
  • Published : 2000.04.08

Abstract

The production of pullulan by Aureobasidium pullulans HP-2001 was investigated under various ratios of glucose as carbon source and yeast extract as the nitrogen source, Highest conversion rate (productivity) of glucose to pullulan was 40.0% when concentrations of glucose and yeast extract were 5% and 0.15%, respectively. Maximal production of pullulan was 29.3g/1 when the concentration of glucose was 8%(w/v) and that of yeast extract was 40:1. On basis of the result that production of pullulan was found in a medium which concentration of glucose as carbon source was up to 20%(w/v), Aureobasidium pullulans HP-2001 seemed to overcome the catabolite repression. Conversion rate of pullulan from 20%(w/v) of glucose was 11.1%.

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