Enhancement of Avermectin Production by Introduction of a Foreign Regulatory Gene in Streptomyces avermitilis

  • 황용순 (서울대학교 응용화학부, 생물공학실험실) ;
  • 이재영 (서울대학교 응용화학부, 생물공학실험실) ;
  • 김상수 (서울대학교 응용화학부, 생물공학실험실) ;
  • 김응수 (한국외국어대학교 환경학과, 환경생물공학 실험실) ;
  • 최차용 (서울대학교 응용화학부, 생물공학실험실)
  • Published : 2000.04.08

Abstract

Production of eight avermectin components was improved in Streptomyces avermitilis wild type strain (ATCC31267) and high producing mutant strain (ATCC31780) when transformed with a foreign regulatory gene, afsR2 of Streptomyces lividans. Wild type and the high producing strain of S. avermitilis transformed with multiple copies of afsR2 improved total avermectin productions by 2.3 fold and 1.5 fold, respectively. In both of wild type and the high producing transformants carrying afsR2, glycerol was proved to be the best carbon source for the stimulation of avermectin production.

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