Optimal Condition for the Production of Exopolysaccharide by Marine Microorganism 96CJ10356

Marine microorganism strain 96CJ10356 produced exopolysaccharides, designated as EPS-R. To optimize culture conditions for the production of EPS-R, carbon, nitrogen, mineral salt, temperature, and pH were examined. STN medium was suggested as follow; sucrose 20, tryptone 10, NaCl 10, $MgSO_4$ 5, $CaCl_2$ 1, $KH_2PO_4$ 0.0076, $K_2HPO_4$ 0.0083, $FeCl_2$ 0.005, $MnCl_2$ 0.001, $NaMoO_4$ 0.001, $ZnCl_2$ 0.001 (g/1) and pH 7.0 About 9.23 g/l of EPS-R was obtained from the STN medium after cultivation for 120 h at $25^{\circ}C$ in 5-liter jar fermentor with an aeration rate of 0.17 vvm. Apparent viscosity and flocculation activity of the culture broth were increased with the production of the EPS-R and the maximal values were reached to 415 cp and 1400 units/ml against 0.5 % activated carbon, respectively.