한국생물공학회:학술대회논문집
- 2000.04a
- /
- Pages.41-44
- /
- 2000
Characterization of two substrates fermentation processes for xylitol production using recombinant Saccharomyces cerevisiae containing xylose reductase gene
- Published : 2000.04.08
Abstract
Fermentation characteristics of recombinant Saccharomyces cerevisiae containing the xylose reductase gene from Pichia stipitis were analyzed in an attempt to convert xylose to xylitol, a natural five-carbon sugar alcohol used as a sweetener. Xylitol was produced with a maximum yield of 0.95 (g xylitol/g xylose consumed) in the presence of glucose that is used as a cosubstrate for cofactor regeneration. However addition of glucose caused inhibition of xylose transport and accumulation of ethanol. Such problems were solved by adopting glucose-limlted fed-batch fermentation. This process done with S, cerevisiae EHl3.15:pY2XR at
Keywords