Proceedings of the Korean Society for Applied Microbiology Conference (한국미생물생명공학회:학술대회논문집)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Phylogenetic Analysis of Bacterial Diversity of Enhanced Biological Phosphorus Removal Activated Sludge by Isolation and Cloning of 16S rDNA

  • Nakamura, Kazunori (National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology) ;
  • Hanada, Satoshi (National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology) ;
  • Kamagata, Yoichi (National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology) ;
  • Kawaharasaki, Mamoru (National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology)
  • Published : 2000.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Bacterial community structure composing enhanced biological phosphorus removal (EBPR) activated sludge was analyzed phylogenetically by cloning 165 rDNA after direct DNA extraction. Then, this result was compared with 165 rDNA sequences of randomly isolated bacterial species. The results clearly showed that there are no coincidence between the sequences retrieved directly from activated sludge and those of isolated strains, suggesting that many important bacteria are hidden in activated sludge because of the difficulty in isolation and culture of them.

Keywords