MASS PRODUCTION OF ENTOMOPATHOGENIC NEMATODE HETERORHABDITIS BACTERIPHORA IN VIVO AND VITRO CULTURE

  • Published : 2000.04.01

Abstract

The strategies of commercial development have been focused on the economy of scale for a process. The design of media has been recognized as a key in assuring mass production of entomopathogenic nematodes. Media optimization was conducted with insect host, proteins, lipids, and symbiotic bacteria mass. G. mellonella (insect host) produced about 290,000 infective juveniles per one. Complex media produced about 250,000 infective juveniles / ml in liquid culture within 8 days (one generation).

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